ZIP14 KO, B6- Slc39a14 KO ZIP14 KO, B6- Slc39a14 KO Heterozygote x Wild-type [C57BL/6JJcl] 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。PLoS One. 2011 6(3):e18059. Heterozygote x Wild-type [C57BL/6JJcl] C（3〜6か月） Zip14 (Slc39a14) 遺伝子のノックアウトマウス。エクソン5から8をneoカセットで置換。Zip14は、Slc39/ZIPファミリー亜鉛トランスポーターに属し、主に骨芽細胞／軟骨細胞／象牙芽細胞／線維芽細胞等に発現する。Zip14遺伝子の機能を阻害すると、ホモマウスはG蛋白共役型受容体シグナル伝達系の支障によって成長遅延と骨代謝異常を呈する。全身成長・骨代謝・糖新生機能に関わる研究に有用である。 <a href='https://brc.riken.jp/mus/pcr06222'>Genotyping protocol -PCR-</a> 小安　重夫 Developmental Biology Research Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C (3-6 months) RBRC06222 Metabolism Research Developed by Toshiyuki Fukuda, RIKEN Center for Allergy and Immunology in 2011. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. C57BL/6 and 129 mixed background. mouse phosphoglycerate kinase (Pgk) promoter, E. coli Neomycin resistance gene, mouse Slc39a13 genomic DNA 深田　俊幸 B6.Cg-Slc39a14<tm1Thir> B6.Cg-Slc39a14<tm1Thir> Zip14 (Slc39a14) gene knockout mice. Exons 5 through 8 were replaced with a neo cassette. Toshiyuki FUKADA M1 [(C57BL/6 x 129)F1] Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 2011 6(3):e18059. 理化学研究所・免疫アレルギー科学総合研究センター・深田俊幸（2011）。M1 ES細胞由来。C57BL/6と129の混合背景。 true Shigeo KOYASU